Composite

Part:BBa_K3748025:Design

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pRNR1-BS-tTDH1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2691
    Illegal EcoRI site found at 1440
    Illegal BglII site found at 705
    Illegal BglII site found at 1912
    Illegal BglII site found at 2082
    Illegal BamHI site found at 1932
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Golden Gate assembly

Source

MoClo Kit and Uniprot

References

Lee, M. E., Deloache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol., 4, 975–986. https://doi.org/10.1021/sb500366v https://www.uniprot.org/uniprot/P00691 - Alpha amylase B. subtilis